John D Cleary,
Assistant Scientist
Department:
Molecular Genetics & Microbiology
Business Phone:
(352) 273-5847
Business Email:
john.cleary@ufl.edu
Research Profile
My research focuses on understanding the disease mechanisms and evaluating therapeutic strategies for the large and growing family of repeat expansion diseases, including myotonic dystrophy (DM), spinocerebellar ataxias (SCAs) and C9orf72 amyotrophic lateral sclerosis (C9-ALS).
Open Researcher and Contributor ID (ORCID)
0000-0001-7599-5995
Areas of Interest
- Genetics
- Molecular therapy
- Myotonic Dystrophy
- Rare neuromuscular disorders
- Repeat Expansion Diseases
Publications
2022
Molecular characterization of myotonic dystrophy fibroblast cell lines for use in small molecule screening.
iScience.
25(5)
[DOI] 10.1016/j.isci.2022.104198.
[PMID] 35479399.
2021
The alternative initiation factor eIF2A plays key role in RAN translation of myotonic dystrophy type 2 CCUG•CAGG repeats.
Human molecular genetics.
30(11):1020-1029
[DOI] 10.1093/hmg/ddab098.
[PMID] 33856033.
2020
Drug Screen Tugs at Common Thread for Repeat Disorders.
Trends in pharmacological sciences.
41(2):71-73
[DOI] 10.1016/j.tips.2019.12.004.
[PMID] 31926601.
2020
The potential of engineered eukaryotic RNA-binding proteins as molecular tools and therapeutics.
Wiley interdisciplinary reviews. RNA.
11(1)
[DOI] 10.1002/wrna.1573.
[PMID] 31680457.
2019
A CTG repeat-selective chemical screen identifies microtubule inhibitors as selective modulators of toxic CUG RNA levels.
Proceedings of the National Academy of Sciences of the United States of America.
116(42):20991-21000
[DOI] 10.1073/pnas.1901893116.
[PMID] 31570586.
2019
Abnormal nuclear aggregation and myotube degeneration in myotonic dystrophy type 1.
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology.
40(6):1255-1265
[DOI] 10.1007/s10072-019-03783-w.
[PMID] 30891637.
2019
Mitigating RNA Toxicity in Myotonic Dystrophy using Small Molecules.
International journal of molecular sciences.
20(16)
[DOI] 10.3390/ijms20164017.
[PMID] 31426500.
2019
Repeat-Associated Non-ATG Translation: Molecular Mechanisms and Contribution to Neurological Disease.
Annual review of neuroscience.
42:227-247
[DOI] 10.1146/annurev-neuro-070918-050405.
[PMID] 30909783.
2018
All in the Family: Repeats and ALS/FTD.
Trends in neurosciences.
41(5):247-250
[DOI] 10.1016/j.tins.2018.03.010.
[PMID] 29703376.
2018
Altered levels of the splicing factor muscleblind modifies cerebral cortical function in mouse models of myotonic dystrophy.
Neurobiology of disease.
112:35-48
[DOI] 10.1016/j.nbd.2018.01.003.
[PMID] 29331264.
2018
Intron retention induced by microsatellite expansions as a disease biomarker.
Proceedings of the National Academy of Sciences of the United States of America.
115(16):4234-4239
[DOI] 10.1073/pnas.1716617115.
[PMID] 29610297.
2018
Repeat-associated non-ATG (RAN) translation.
The Journal of biological chemistry.
293(42):16127-16141
[DOI] 10.1074/jbc.R118.003237.
[PMID] 30213863.
2018
Therapeutic Genome Editing for Myotonic Dystrophy Type 1Â Using CRISPR/Cas9.
Molecular therapy : the journal of the American Society of Gene Therapy.
26(11):2617-2630
[DOI] 10.1016/j.ymthe.2018.09.003.
[PMID] 30274788.
2017
Impeding Transcription of Expanded Microsatellite Repeats by Deactivated Cas9.
Molecular cell.
68(3):479-490.e5
[DOI] 10.1016/j.molcel.2017.09.033.
[PMID] 29056323.
2017
New developments in RAN translation: insights from multiple diseases.
Current opinion in genetics & development.
44:125-134
[DOI] 10.1016/j.gde.2017.03.006.
[PMID] 28365506.
2017
RAN Translation Regulated by Muscleblind Proteins in Myotonic Dystrophy Type 2.
Neuron.
95(6):1292-1305.e5
[DOI] 10.1016/j.neuron.2017.08.039.
[PMID] 28910618.
2014
Repeat associated non-ATG (RAN) translation: new starts in microsatellite expansion disorders.
Current opinion in genetics & development.
26:6-15
[DOI] 10.1016/j.gde.2014.03.002.
[PMID] 24852074.
2013
Repeat-associated non-ATG (RAN) translation in neurological disease.
Human molecular genetics.
22(R1):R45-51
[DOI] 10.1093/hmg/ddt371.
[PMID] 23918658.
2010
Repeat instability as the basis for human diseases and as a potential target for therapy.
Nature reviews. Molecular cell biology.
11(3):165-70
[DOI] 10.1038/nrm2854.
[PMID] 20177394.
2010
Tissue- and age-specific DNA replication patterns at the CTG/CAG-expanded human myotonic dystrophy type 1 locus.
Nature structural & molecular biology.
17(9):1079-87
[DOI] 10.1038/nsmb.1876.
[PMID] 20711191.
2008
CTCF cis-regulates trinucleotide repeat instability in an epigenetic manner: a novel basis for mutational hot spot determination.
PLoS genetics.
4(11)
[DOI] 10.1371/journal.pgen.1000257.
[PMID] 19008940.
2005
Repeat instability: mechanisms of dynamic mutations.
Nature reviews. Genetics.
6(10):729-42
[PMID] 16205713.
2005
Replication fork dynamics and dynamic mutations: the fork-shift model of repeat instability.
Trends in genetics : TIG.
21(5):272-80
[PMID] 15851063.
2003
The contribution of cis-elements to disease-associated repeat instability: clinical and experimental evidence.
Cytogenetic and genome research.
100(1-4):25-55
[PMID] 14526163.
2002
Evidence of cis-acting factors in replication-mediated trinucleotide repeat instability in primate cells.
Nature genetics.
31(1):37-46
[PMID] 11967533.
2002
In vitro (CTG)*(CAG) expansions and deletions by human cell extracts.
The Journal of biological chemistry.
277(16):13926-34
[PMID] 11832482.
2002
Slipped-strand DNAs formed by long (CAG)*(CTG) repeats: slipped-out repeats and slip-out junctions.
Nucleic acids research.
30(20):4534-47
[PMID] 12384601.
2000
Nucleotides of the tRNA D-stem that play an important role in nuclear-tRNA export in Saccharomyces cerevisiae.
The Biochemical journal.
347 Pt 1:115-22
[PMID] 10727409.
Contact Details
Phones:
- Business:
- (352) 273-5847
Emails:
- Business:
- john.cleary@ufl.edu
Addresses:
- Business Mailing:
-
PO BOX 103610
GAINESVILLE FL 326100001 - Business Street:
-
ROOM 225
PO BOX 103610
CANCER AND GENETICS RESEARCH COMPLEX
GAINESVILLE FL 32608